Overview

The Mag-Bind® Environmental DNA 96 Kit allows rapid and reliable isolation of high-quality DNA from soil and water samples. The Mag-Bind® Environmental DNA 96 Kit can isolate microbial DNA from yeast, fungi, and gram-positive or negative bacteria. Up to 96 one hundred mg soil samples can be processed in 120 minutes using automated liquid handlers or magnetic processors. Omega Bio-tek’s unique cHTR Reagent effectively removes humic acid and other PCR inhibitors allowing for purified DNA to be suitable for PCR, 16S sequencing, Whole Genome Sequencing, and Next-Generation Sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel. 

Product Data

DNA from soil samples using Mag-Bind® Environmental Kit had higher yield and quality, less inhibitor than using a leading competitor’s product

Figure 1.  Outdoor soil (250mg) was spiked with an approximately 1mL culture of Bacillus subtilis and DNA was extracted using Mag-Bind® Environmental kit (Omega Bio-tek) and Soil DNA Isolation kit (Company Q) following manufacturer’s recommended protocols. The isolations were carried out in quadruplicate for the Omega kit and in duplicate for the competitor’s kit. DNA was quantified using Thermo Scientific’s NanoDrop® 2000c. The quality of the purified DNA was analyzed by performing real-time PCR using Bacillus subtilis specific primers on undiluted and 10-fold diluted DNA isolated from soil extraction protocol.

qPCR Comparison From Water Samples

Figure 2.  Pond water (100mL) was spiked with approximately 1mL culture each of B. subtilis and E. coli harboring pGEM plasmid and was filtered through Sterivex (Millipore SVGPL10RC) filters. Upon filtration, DNA was extracted from these filters in quadruplicate following the manufacturer’s recommended protocols. DNA was quantified using Thermo Scientific’s NanoDrop® 2000c. The quality of the purified DNA was analyzed by performing real-time PCR using Bacillus subtilis specific primers on 10-fold diluted and 100-fold diluted DNA isolated from water extraction protocol. 

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