Application

This kit can be used to measure Glucose (Glu) content in serum, plasma samples. 

Detection significance

It is very important for diagnosis of hyperglycemia to accurate determination of glucose. Usually, there is also a variety of inhibition test and determination of glucose tolerance test at the same time with glucose measuring during finding the cause of these conditions. Glucose level increases seen in diabetes mellitus, glucose intake, cushing syndrome and cerebrovascular accident. Glucose content decreases seen in insulinoma, insulin overdose and congenital carbohydrate metabolism disorder.

Detection principle

Glucose oxidase can catalyze the oxidation of glucose to gluconic acid to produce hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide and oxidizes pigment sources to form colored substances. Measure the OD value at 505 nm and glucose content can be calculated indirectly.

Experimental instrument

Spectrophotometer (505 nm), Pipettor, Water bath, Centrifuge

Sample preparation

Serum (Plasma): Detect directly. Or dilute the sample with normal saline if the result exceeds the detection range of the kit (0.05~30 mmol/L).

Note: Serum or plasma should be isolated from the sample tube as soon as possible without hemolysis. Serum or plasma can be stored at 2~8℃ for 24h.

Operation steps

Measured by spectrophotometer

Reference value

Normal human serum (plasma): 3.89-6.11 mmol/L (70-110 mg/dL). This is for reference only. It is recommended that each laboratory establishes its own reference value range.

Limitations of method

When the test result is more than 30 mmol/L, please dilute the sample with normal saline, and then multiply the dilution multiple in calculation

Technical parameter

1. The sensitivity of the kit is 0.05 mmol/L.

2. The intra-assay CV is 1.2 % and the inter-assay CV is 1.3%.

3. The recovery of the kit is 101 %.

4. The detection range of the kit is 0.05-30 mmol/L.

Notes

1. This kit is for research use only.

2.  Please progress strictly with operation procedures.

3. Do not use components from different batches of kit.

4. The validity of kit is 6 months.

5. There may be a certain stimulative effect or toxicity while the reagents containing preservatives and stabilizer, please do not directly contact the skin and eyes. Rinsing with abundant water once the contact and do not swallow.

6. Separate serum or plasma from red blood cell immediately after blood collection to avoid the glycolysis. It can prevent the glycolysis within 24 hours if adding sodium fluoride.

7. Ensure the accuracy and reliability of the results, the quality control must be carry in each batch.