The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.
The E.Z.N.A.® Plasmid Mini Kit is available in two formats – Q-spin (D6942) and V-spin (D6943). D6942 (Q-spin) features columns that are capless whereas D6943 (V-spin) includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream Application | Cloning; sanger sequencing etc. |
| Starting material | 5-15 mL LB culture with OD600 between 2 and 3; or equivalent |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing mode | Manual, centrifugation or vacuum |
| Throughput | 1 - 24 |
| DNA binding technology | Silica Mini Spin Column II (2x capacity of mini spin column) |
| Lysate clearance method | Centrifugation |
| Processing time | <30 minutes |
| Yield | 40-70 µg for high copy-number; 1-15 µg for low copy-number |
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