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Overview
By using a unique MicroElute spin column, the E.Z.N.A.® MicroElute Gel Extraction Kit is designed for the purification of DNA fragments from agarose gels with a small elution volume of 10-15 µL.
- Elution volumes – 10-15 µL
- Generation of highly concentrated DNA
- 10 µg DNA binding capacity
- Rapid isolation – less than 15 minutes
Specifications
For Research Use Only. Not for use in diagnostic procedures.
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream application | Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 30-50 µL |
| Starting material | Agrose gel slice |
| Starting amount | Up to 25 µg DNA |
| DNA recovered | >90% recovery, 70 bp to 20 kb |
| Processing mode | Manual, centrifugation/vacuum |
| Throughput | 1-12 |
| DNA binding technology | Silica mini spin column |
| Binding capacity | 25 μg |
| Processing time | <10 minutes |
Kit Components
| ITEM | AVAILABLE SEPARATELY |
|---|---|
| HiBind® DNA Mini Columns | View Product |
| 2 mL Collection Tubes | View Product |
| Binding Buffer (XP2) | View Product |
| Elution Buffer | View Product |
| SPW Wash Buffer | View Product |
Product Data
Electrophoretic Analysis of recovered DNA from an agarose gel compared to the control
Figure 1. DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q following manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being compared to the original amount used in the gel extraction procedure. M: size marker; C: control; O: Omega Bio-tek; Q: company Q.
Comparison of DNA fragment recovery from an agarose gel
Figure 2. DNA was purified from an agarose gel using Omega Bio-tek’s E.Z.N.A.® Gel Extraction Kit and a comparable kit from Company Q. The concentration of the recovered DNA was determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. The purified DNA normalized to input amount is shown above.
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